Antibacterial Activity Test of Cat's Whiskers Leaf Extract (Sauropus androgynus (L) Merr) Serum Preparation Against Propionibacterium acnes Bacteria
DOI:
https://doi.org/10.56480/pratamamedika.v2i1.939
Abstrak View:
327
PDF downloads:
283
Kata Kunci:
Serum, Katuk Leaves Extract, Propionibacterium AcnesAbstrak
One of the inflammatory diseases that occurs in the pilosebaceous unit is acne. One of the bacteria that causes acne is Propionibacterium acnes. Katuk leaves contain flavonoids, saponins, tannins and alkaloids which can be used as an acne treatment. One of the pharmaceutical preparations that can be used to treat acne is serum. The aim of this research is to formulate katuk leaf extract into a serum preparation, determine the physical quality characteristics of the serum preparation and then determine the antibacterial activity against Propionibacterium acnes.The research method used is experimental. Katuk leaves were extracted by maceration method with 70% ethanol then serum preparations were made with various extract concentrations, namely FI 40%, FII 60% and FIII 80% on the basis of serum xanthan gum. The physical quality test of the serum preparation including organoleptic, pH, homogeneity, spreadability, adhesion and viscosity was carried out, then the antibacterial activity test of Propionibacterium acnes was carried out with positive control of clindamycin 10 µg/disk, negative control of 5% DMSO using disc diffusion method. The results of this study indicate that the formula has a semi-solid form, the color of FI is brownish green, FII is dark green and FIII is blackish green with a distinctive aroma of the extract, the formulation is homogeneous, has a pH, spreadability, adhesion and viscosity that meets the requirements of a good serum. The results of the antibacterial activity test showed that the negative control had no inhibition zone, the positive control showed a strong inhibition zone of 20.09 and the katuk leaf extract serum formulation had a moderate inhibition zone of FI 18.11, FII 19.21 and FIII 19.36 the greater the inhibition zone produced due to the higher concentration of the extract made. The results of statistical analysis of variations in extract concentrations showed significant differences in the inhibition zone based on One Way Anova statistical analysis (p<0.05).
Referensi
Afifah, Aninda Nur. 2019. Pengertian Micellar Based Water Minyak Biji Kelor dengan Sentralisasi Berbeda Pasak 7 Gliseril Cocoate Sebagai Surfaktan. Komposisi Logis Postulasi, Perguruan Tinggi Setia Budi Surakarta. http://repository.setiabudi.ac.id/id/eprint/3709
Amira, KJ (2021). Pengertian Susunan Serum dari Konsentrat Belimbing wuluh (Averrhoa Bilimbi L.) Terhadap Mikroba Propionibacterium Acnes Secara In Vitro (Doctoral Paper, Stikes Karya Putra Bangsa Tulungagung).
Andini, D. 2014. Kemampuan Daun Katuk (Sauropus androgynus L. Merr) sebagai Ramuan Cinta. Diary Klinis Perguruan Tinggi Lampung, 7 (3): 17-22.
Andini, T., Yusriadi, Y., Yuliet, Y., 2017. Peningkatan Pengembangan Film Minuman Keras Polivinil dan Humektan Propilen Glikol pada Persamaan Strip Off Gel Cover Jus Labu Kuning (Cucurbita moschata Duchesne) sebagai Agen Pencegah Kanker. Galenika Diary Toko Obat (e-Diary), 3(2), 165-173.
Anggraeni, O.N., Fasya, A.G., Abidin, M., dan Hanapi, A. 2014. Uji Coba Aksi Agen Pencegah Kanker Derivasi Asam Etil Asetat, Divisi Kloroform, Petrol Eter, dan n-Hexane Terjadi Karena Hidrolisis Konsentrat Metanol dari Mikroalga Klorella sp. Kimia spekulatif, 3(2).
Anwar, S dan Nurelilasari S, 2020. Skrining Fitokimia Daun Katuk (Sauropus androgynus L) Sebagai Pemicu ASI. Buku Harian Logis Kesejahteraan Indonesia. Jil.5 (1): 85-89.
Arianti J, R., 2017, Pengertian dan Uji Ketergantungan Sebenarnya Gel Anti Peradangan Kulit dari Konsentrat Etanol Strip Pisang Ambon Muda. Proposal Perguruan Tinggi Islam Negeri Alauddin, hal.1–2.
Arisanty, A., dan Dewi, RP (2018). Uji Coba Kecukupan Konsentrat Air Produk Bintang Organik (Averrhoa bilimbi) terhadap Perkembangan Propionibacterium acnes. Media Narkoba, 14(2), 66-71.
Aryani, N. T., Kawiji, Khasanah, L. U. terlebih lagi, Utami, R. 2015. Ekstraksi Oleoresin Maserasi Daun Jeruk Nipis (Citrus hystrix): Pengefisienan Hasil dan Pengujian Mutu Nilai. Buku Harian Agritech, 35(2), 178-184.
Berawi, K. N., Marini, D., Fisiologi, B., Pengobatan, F., Lampung, U., Dokter, M. P., Pengobatan, F., dan Lampung, U. 2018. Viabilitas Kulit Pohon Bakau Minyak (Rhizopora apiculata) sebagai a Penguat Sel Viabilitas Kulit Kayu Rhizopora apiculata sebagai Penguat Sel. 5, 412–417.
BPOM, R. I. 2014. Pedoman Pimpinan Badan Pengawas Obat dan Makanan Republik Indonesia Nomor 12 Tahun 2014 tentang Prasyarat Mutu Obat Konvensional. BPOM RI, halaman 3, Jakarta.
Budiana, W., Nuryana, E.F., Suhardiman, A., dan Kusriani, H. (2022). Tindakan pencegahan kanker daun katuk (Breynia androgyna L.) terpisah dengan teknik DPPH dan jaminan kadar fenolat dan flavonoid. Buku Harian Ummat Agrotech, 9(4), 275-286.
Pelayanan Kesehatan Republik Indonesia. Tahun 2020. Keluaran Farmakope Indonesia VI. Pelayanan Kekuatan Republik Indonesia. Jakarta.
Desnita, R dkk. 2018. Aksi Penenangan Memperbaiki Konsentrat Etanol Daun Katuk (Sauropus Androgynus L. Merr). Buku Harian Ilmu Obat Indonesia Vol.16 No.1:1-5.
Emelda. 2019. Farmakognosi : Untuk Mahasiswa Kemampuan Kemampuan Penyimpan Obat. Yogyakarta: Pustaka Baru Pers.
Emma S dan Pratiwi D. 2014. Pengkajian dan Uji Coba Kesehatan Aktual dan Sineresis Susunan Gel yang Mengandung Minoxidil, Apigenin dan Sari Rempah Seledri (Apium Graveolens L.). Diary Rilisan Eksplorasi Kesejahteraan Indonesia, 42(4), hal. 20088.
Fadilah, N.N., Agustien, G.S., dan Rizkuloh, L.R. (2022). Uji Coba Aksi Antidiare Konsentrat Etanol Daun Katuk (Breynia androgyna (L.)) pada Mencit Putih Memanfaatkan Teknik Perjalanan Gastrointestinal. Fasilitas Penyimpanan Obat: Diary of Drug Sciences, 3(2), 331-340.
Ferdy F, Reihan K, Wildan K. M dan Lutfi C. 2022. Uji Coba Aksi Antibakteri Konsentrat Etanol Serum Produk Organik Star Terhadap Propionibacterium acnes, Staphylococcus aureus dan Staphylococcus epidermis. Toko obat dan Majalah Farmakologi. MFF 2022; 26(2): 69-73, DOI: 10.20956/mff.v26i2.18578.
Fissy, O.N., Sarim R., dan Pratiwi, L. 2014. Kemanjuran Gel Melawan Jerawat Kulit dari Konsentrat Etanol Rimpang Jahe Merah (Zingiber officinale Rosc. Var. Rubrum) Terhadap Propionibacterium acnes dan Staphylococcus epidermidis. Buku Harian Ilmu Obat Indonesia 12 (2): 194-201.
Fitriana WD, Ersam T, Shimizu K, dkk. Pergerakan agen pencegahan kanker dari Moringa oleifera memisahkan. J Kimia Indonesia. 2016; 16: 297–301.
Hafsari, R.A. et al. Trial of the Antibacterial Action of Beluntas Leaf Concentrate (Pluchea indica (L.) Less) Against Propionibacterium Acnes, the Reason for Skin inflammation. Issn. 2015 (1):141–61.
Hasrawati A., Hardianti., Qama A., and Wais M, 2020, Improvement of Papaya Seed Squander Ethanol Concentrate (Carica papaya L.) as an Enemy of Skin Inflammation Serum. Indonesian Phytopharmaca Diary. 7(1), pp. 1–8.
Hidayah N. 2016. Movement Trial of Methanol Concentrate of Klika Dara (Croton Oblongus Burm F) Against Skin break out Causing Microscopic organisms. Thesis. Undergrad Drug Store Study Program, Workforce of Medication and Wellbeing Sciences UIN Alauddin, Makassar, pp. 1–68.
Hikmawati N.P.E and Fatmawati, S. 2019. Free Exploration on the Potential Cell strengthening Action of Some Katuk (Sauropus Androgynus (L.) Merr.) Leaf Concentrates Against DPPH Free Extremists. Autonomous Exploration. Drug store Study Program, Staff of Drug store and Science, Muhammadiyah College, Prof. Dr. Hamka.
Hawk Eng Khoo, A. A. furthermore, A. I. 2015. Sauropus androgynus Leaves for Medical advantages: Publicity and the Science. The Regular Items Diary, 5(2), 115-123. https://doi.org/10.2174/221031550502150702142028
Ikhsanudin, A., and Mardhiyah, S. 2017. Definition and Testing of Against Skin inflammation Gel with 70% Ethanol Concentrate of Star Organic product (Averrhoa Bilimbi Linn.) Against Propionibacterium Acnes Microorganisms. MEDULA, 5(1).
Indriyati W, Kusmawati R, Sriwidodo S, Hasanah An, Musfiroh I. 2017. Portrayal of Carboxymethyl Cellulose Sodium (Na-Cmc) from Water Hyacinth Cellulose (Eichhornia crassipes (Store.) Solms.) Filling in the Jatinangor and Lembang Regions. Indonesia J Pharm Sci Technol. 3(3):99.
Juliastuti. 2019. Viability of Katuk Leaves on the Ampleness of Bosom Milk in Breastfeeding Moms at the Kuta Baro Wellbeing Center, Aceh Besar. Indonesian Diary For Wellbeing Sciences Vol. 3 No. 01, 1-5.
Kalangi, S. J. 2013. Skin Histophysiology. Manado: Workforce of Medication, Sam Ratulangi College.
Kartikasari, D., Justicia, A. K., and Endang, P. 2019. Assurance of Complete Flavonoid Levels in Ethanol Concentrates of Red Andong Leaves and Green Andong Leaves. Indonesian Drug Human Diary, 2(1), 108-117. https://e-jurnal.stikes-isfi.ac.id/index.php/JIFI/article/view/302.
Syamsul A. 2015. Test of the Antibacterial Activity of Basil Leaf Extract (Ocimum sanctum L) in Gel Dosage Form. Thesis. 1–98.
Wahyuningsih, S., Auliah, N., and Salwi, S. 2020. Pineapple (Ananas comosus L. Merr) Juice Mouthwash Against Streptococcus mutans Bacteria. Journal of Health, 13(2), 171.
Wamida, H., Juliannor, A., Sukawati, Y. 2016. Formulation of Dayak Onion (Eleutherine bulbosa (Factory.) Urb.) Extract into Gel Toothpaste. Journal of Pharmaceutical and Clinical Sciences, 3(1), 42-49.
Wiendarlina IY., Rahminiwati M., Gumelar FT., . Hepatoprotective Activity of Water Extract of Small Leaf Gotu Kola Herb (Centella asiatica L. Urb.) Against Male White Rats Sprague Dawley L. Induced with Paracetamol. Phytopharmaca Pharmaceutical Scientific Journal. 2019;8(1):12–22.
Wijaya H., Novitasari. and Jubaidah S. 2018. Comparison of Extraction Methods on the Yield of Sea Hair Leaf Extract (Sonneratia caseolaris L. Engl). Manuntung Scientific Journal, 4(1), pp.79-83.
Winarsih, et al. 2015. Antibacterial Effect of Katuk (Sauropus androgynus) Leaf Extract on the Growth of Salmonella Typhi In Vitro. Mutiara Medika. Vol. 15 Numbers 2:96-103.
Yusriyani, Y. (2019). Test of the Antibacterial Activity of Ethanol Extract of Katuk Leaves (Sauropus Androgunus (L) Merr) Against the Growth of Staphylococcus Aureus and Escherichia Coli Using Bioautography. Yamasi Makassar Health Journal, 3(2).
Zukhri, S., Dewi, K.M., and Hidayati, N. (2018). Test of the Physical and Antibacterial Properties of Katuk Leaf Extract Ointment (sauropus androgynus (l) merr.).
Unduhan
Diterbitkan
Terbitan
Bagian
Lisensi
Hak Cipta (c) 2023 Maulina Nur Riskiani, Gigih Kenanga Sari, Maulita Saraswati
Artikel ini berlisensiCreative Commons Attribution-ShareAlike 4.0 International License.
Copyright Notice
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
